AKT1, AKT2, AKT3 Antibody

Rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1 Antibody, AKT2 Antibody, AKT3 Antibody

Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

AKT Antibody is tested in western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Expect bands at ~56 kDa in size corresponding to AKT by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.