GAB1 Antibody

Rabbit Anti-GAB1 Antibody, GAB 1, GAB-1, GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1

Anti-GAB1 Antibody was produced in rabbits by repeated immunizations with a recombinant protein produced in Sf9 cells corresponding to the full length human GAB protein.

Anti-GAB1 antibody is directed against human GAB1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-GAB1 antibody was purified from rabbit serum by Protein A chromatography followed by cross adsorption. Cross reactivity with GAB1 from other species has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-GAB1 Antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at 76.6 kDa by western blotting in the appropriate cell lysate or extract.