KLH Antibody
KLH antibody
Product Specifications
Product Name Alternative
Mouse anti-KLH Antibody, Keyhole Limpet Hemocyanin, KLH antibody
Reactivity
Other
Immunogen
Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
Clonality
Monoclonal
Clone
3B1
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-KLH has been prepared from mouse ascitic fluid by immunoaffinity chromatography. KLH antibody is specific for KLH. Does not cross react with mouse heart or skin tissue sections. Cross-reactivity from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-KLH can be used as an IgM isotype control in various assays or in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype
IgM
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