KLH Antibody

Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.

Anti-KLH has been prepared from mouse ascitic fluid by immunoaffinity chromatography. KLH antibody is specific for KLH. Does not cross react with mouse heart or skin tissue sections. Cross-reactivity from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-KLH can be used as an IgM isotype control in various assays or in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.