BB-H32, bba64 Antibody

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.