Anti-Synaptotagmin-7 Antibody

Cell membrane, Cell junction, synapse, presynaptic cell membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Lysosome membrane, Cytoplasmic vesicle, phagosome membrane, Peroxisome membrane, Cytoplasmic vesicle, secretory vesicle membrane

Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory and synaptic vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. SYT7 binds Ca(2+) with high affinity and slow kinetics compared to other synaptotagmins (PubMed:26738595). Involved in Ca(2+)-triggered lysosomal exocytosis, a major component of the plasma membrane repair (By similarity). Ca(2+)-regulated delivery of lysosomal membranes to the cell surface is also involved in the phagocytic uptake of particles by macrophages (PubMed:16982801, PubMed:21041449). Ca(2+)-triggered lysosomal exocytosis also plays a role in bone remodeling by regulating secretory pathways in osteoclasts and osteoblasts (PubMed:18539119). Involved in cholesterol transport from lysosome to peroxisome by promoting membrane contacts between lysosomes and peroxisomes: probably acts by promoting vesicle fusion by binding phosphatidylinositol-4,5-bisphosphate on peroxisomal membranes (PubMed:25860611). Acts as a key mediator of synaptic facilitation, a process also named short-term synaptic potentiation: synaptic facilitation takes place at synapses with a low initial release probability and is caused by influx of Ca(2+) into the axon terminal after spike generation, increasing the release probability of neurotransmitters (PubMed:24569478, PubMed:26738595). Probably mediates synaptic facilitation by directly increasing the probability of release (PubMed:26738595). May also contribute to synaptic facilitation by regulating synaptic vesicle replenishment, a process required to ensure that synaptic vesicles are ready for the arrival of the next action potential: SYT7 is required for synaptic vesicle replenishment by acting as a sensor for Ca(2+) and by forming a complex with calmodulin (PubMed:24569478). Also acts as a regulator of Ca(2+)-dependent insulin and glucagon secretion in beta-cells (PubMed:18308938, PubMed:19171650). Triggers exocytosis by promoting fusion pore opening and fusion pore expansion in chromaffin cells (PubMed:20956309). Also regulates the secretion of some non-synaptic secretory granules of specialized cells (By similarity). {UniProtKB:Q62747, PubMed:16982801, PubMed:18308938, PubMed:18539119, PubMed:19171650, PubMed:20956309, PubMed:21041449, PubMed:24569478, PubMed:25860611, PubMed:26738595}.

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~45kDa. Other isoforms of ~65kDa are also detected. <br><br>Positive control: rat brain lysate. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N terminal trans- membrane region (TMR), a variable linker,and two C-terminal C2 domains: C2A and C2B. There are 15 members in the mammalian synaptotagmin family. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to act as Ca+2 sensors in vesicular trafficking and exocytosis. Calcium binding to synaptotagmin triggers neurotransmitter release at the synapse. The first C2 domain mediates Ca+2-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.