Methylene Blue

Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene Blue is a photosensitizer and redox agent. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3][4].

Product Specifications

CAS Number

[61-73-4]

Product Name Alternative

Basic Blue 9; CI-52015; Methylthioninium (chloride)

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Guanylate Cyclase; Microtubule/Tubulin; Monoamine Oxidase; NO Synthase

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Cytoskeleton; GPCR/G Protein; Immunology/Inflammation; Neuronal Signaling

Applications

Cancer-programmed cell death

Field of Research

Cancer; Infection; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Methylene-Blue.html

Purity

98.0

Solubility

H2O : 50 mg/mL (ultrasonic)

Smiles

CN(C)C1=CC=C2C(SC(C(C=C/3)=N2)=CC3=[N+](C)/C)=C1.[Cl-]

Molecular Formula

C16H18ClN3S

Molecular Weight

319.85

Precautions

H302, H315, H319, H335

References & Citations

[1]Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94 (2) :65-72. |[2]Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25.|[3]Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32 (2) :127-38.|[4]Kalaitzakis D, et al. Methylene Blue as a Photosensitizer and Redox Agent: Synthesis of 5-Hydroxy-1H-pyrrol-2 (5H) -ones from Furans. Angew Chem Int Ed Engl. 2015 May 18;54 (21) :6283-7.