SARS S protein (R667A, K968P, V969P), His Tag (MALS verified)

SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293) . It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P) ) . The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAP13567.1 (R667A, K968P, V969P)

Expression Region

Ser 14 - Pro 1195

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).