Rabbit anti Human Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)

Endothelial Cell Differentiation Gene-8 (EDG-8) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-8 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-8 is expressed in oligodendrocytes and fibrous astrocytes in the rat brain.

Detects recombinant EDG8 receptors by Western blot (5-10 µg/mL) at 42 kDa. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

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