Phospho-Wee1 (S123) Antibody [APR05159G]
Product Specifications
Synonyms
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Gene Name
WEE1
Gene ID
7465
UniProt
P30291
Chromosomal Location
Nucleus.
Applications
DB, IHC-P
Dilution
DB--1:500 IHC-P--1:100~500
Form
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Molecular Weight
71597
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Overview
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-Wee1 (S123) Antibody [APR05159G].
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/?term=7465
Uniprot URL
https://www.uniprot.org/uniprot/P30291
Available Sizes
Curated Selection
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