Recombinant Human S100B protein

Product Specifications

Target

SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DNDGDGECDF QEFMAFVAMV TTACHEFFEH E

Purification

> 95 % by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug of rHuS100B as determined by LAL method.

Bioactivity

Measured in a cell proliferation assay using human peripheral blood monocytes. The ED50 for this effect is 1-4 μg/mL.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids

Formulation

Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4.

Host or Source

Escherichia coli