GIT1 Antibody (Center) [APR16145G]
Product Specifications
Synonyms
ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1
Gene Name
GIT1
Gene ID
28964
UniProt
Q9Y2X7
Chromosomal Location
Cytoplasm. Cell junction, synapse. Cell junction, synapse, presynapse {ECO:0000250|UniProtKB:Q9Z272}. Cell junction, synapse, postsynapse {ECO:0000250|UniProtKB:Q9Z272}. Cell junction, synapse, postsynaptic density {ECO:0000250|UniProtKB:Q9Z272}. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytosolic complexes, containing at least PXN/paxillin, ARHGEF7 and PAK1, and membrane protrusions. During cell migration, moves from the disassembling adhesions into the cytosol and towards the leading edge. In adherent cells, localizes to adhesions. Recruitment to adhesions may be mediated by RAC and active tyrosine-phosphorylated PXN (PubMed:11896197) . May be present in both excitatory and inhibitory synapses. In hippocampal neurons, recruitment of GIT1 to synapses is regulated by ephrinB activation and ephrinB downstream effector GRB4/NCK2. In hippocampal neurons, partially colocalizes with PCLO (By similarity) . Interaction with GRIN3A limits GIT1 synaptic localization (By similarity) . Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601) {ECO:0000250|UniProtKB:Q9Z272, ECO:0000269|PubMed:11896197, ECO:0000269|PubMed:27012601}
Applications
IHC-P, WB
Dilution
WB--1:2000 IHC-P--1:100~500
Form
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Function
2701260123108400109381121189619715923189126955021580019312695502252847833150230219273721
Molecular Weight
84341
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Overview
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GIT1 Antibody (Center) [APR16145G].
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/?term=28964
Uniprot URL
https://www.uniprot.org/uniprot/Q9Y2X7
Available Sizes
Curated Selection
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