Sudan IV

Sudan IV is an agonist of the aryl hydrocarbon receptor (AhR) that activates downstream signaling pathways and induces CYP1A1 expression. Sudan IV promotes CYP1A1 gene transcription by activating AhR-ARNT heterodimers and binding to exogenous response elements (XREs) on DNA, thereby enhancing drug metabolizing enzyme activity. Sudan IV can be used to study the toxicity mechanisms of industrial dyes and the effects of interactions with serum proteins (such as bovine serum albumin (BSA) ) on their distribution in vivo. Sudan IV is a fat-soluble diazo dye that can be used to stain lipids, triglycerides, and lipoproteins on frozen sections[1][2][3][4].

Product Specifications

CAS Number

[85-83-6]

Product Name Alternative

Solvent Red 24; C.I. 26105

UNSPSC

12171500

Hazard Statement

H317

Target

Aryl Hydrocarbon Receptor; Biochemical Assay Reagents; Cytochrome P450

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; Others

Field of Research

Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/sudan-iv.html

Purity

98.0

Solubility

DMSO : < 1 mg/mL

Smiles

OC1=CC=C2C=CC=CC2=C1/N=N/C3=CC=C(/N=N/C4=CC=CC=C4C)C=C3C

Molecular Formula

C24H20N4O

Molecular Weight

380.44

Precautions

H317

References & Citations

[1]Refat NA, et al. The induction of cytochrome P450 1A1 by sudan dyes. J Biochem Mol Toxicol. 2008 Mar-Apr;22 (2) :77-84.|[2]Lu D, et al. Binding of Sudan II and Sudan IV to bovine serum albumin: comparison studies. Food Chem Toxicol. 2011 Dec;49 (12) :3158-64.|[3]Huang ST, et al. An ultrasensitive and selective fluorescence assay for Sudan I and III against the influence of Sudan II and IV. Biosens Bioelectron. 2013 Apr 15;42:136-40.|[4]Pérez-Sanz F, Riquelme-Pérez M, Martínez-Barba E, de la Peña-Moral J, Salazar Nicolás A, Carpes-Ruiz M, Esteban-Gil A, Legaz-García MDC, Parreño-González MA, Ramírez P, Martínez CM. Efficiency of Machine Learning Algorithms for the Determination of Macrovesicular Steatosis in Frozen Sections Stained with Sudan to Evaluate the Quality of the Graft in Liver Transplantation. Sensors (Basel) . 2021 Mar 12;21 (6) :1993.