ATP4B Antibody (N-term) [APR10860G]

Product Specifications

Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP4B Antibody (N-term) [APR10860G].

Synonyms

Potassium-transporting ATPase subunit beta, Gastric H (+) /K (+) ATPase subunit beta, Proton pump beta chain, ATP4B

Gene Name

HGNC:820

Gene ID

496

UniProt

P51164

Chromosomal Location

Apical cell membrane {ECO:0000250|UniProtKB:P20648}; Single-pass type II membrane protein. Cell membrane {ECO:0000250|UniProtKB:P18597}; Single- pass type II membrane protein. Note=Localized in the apical canalicular membrane of parietal cells {ECO:0000250|UniProtKB:P20648}

Applications

WB, FC

Dilution

WB--1:1000 FC--1:10~50

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Function

The beta subunit of the gastric H (+) /K (+) ATPase pump which transports H (+) ions in exchange for K (+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity) . Within a transport cycle, the transfer of a H (+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2) . Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity) .