Anti-Glutamate NMDA Receptor NR3A/B Subunit Antibody

Mouse Monoclonal Antibody specific to Glutamate NMDA Receptor NR3A/B Subunit

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GluN3A, N-methyl-D-aspartate receptor subtype 3A, NMDAR3A, NR3A, NMDAR-L

Gene Name

GRIN3A

NCBI Gene ID

UniProt

Q8TCU5

Cellular Locus

Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell junction, synapse, postsynaptic density

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 780-914 of the NR3A extracellular III-IV loop

Target Antigen

Glutamate receptor ionotropic, NMDA 3A

Target

Glutamate NMDA Receptor NR3A/B Subunit

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. During the development of neural circuits, plays a role in the synaptic refinement period, restricting spine maturation and growth. By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses. May also play a role in PPP2CB-NMDAR mediated signaling mechanism. {UniProtKB:Q9R1M7}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. Predicted molecular weight 125-130kDa. Detection of NR3A- and NR3B-transfected HEK293T cell lysates probed with #60100. Immunohistochemistry: use at 1-10ug/mL. Mouse spinal cord ventral horn stained with #60100. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This product is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

This antibodyThis antibody recognizes a unique epitope within the immunoglobulin (Ig)-like domain of arginine-gingipain (RgpB). recognizes NMDAR subunits NR3A and NR3B. It does not cross-react with subunits NR1 or NR2A-D.