Biotinylated Human CD81 Protein, His, Avitag™&Flag Tag (Detergent)

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Product Specifications

Background

Specifications

This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™) . The protein has a calculated MW of 31.4 kDa. The protein migrates as 27-29 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

P60033

Expression Region

Gly 2 - Tyr 236

Host

Insect Cells

Target

CD81

Conjugation

Biotin-labeled

Tag

N-Flag & C-10xHis & C-Avi

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 12 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

60%

Bioactivity

Immobilized Biotinylated Human CD81 Protein, His,Avitag&Flag Tag (Cat. No. CD1-H85D8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD81 antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).