EcoPlexTM Human T Helper Cytokine 14-Plex Panel Kit

GM-CSF:P04141 TNF-α:P01375 IL-2:P60568 IFN-γ:P01579 IL-9:P15248 IL-12p70:P29459&P29460 IL-4:P05112 IL-5:P05113 IL-6:P05231 IL-17A:Q16552 IL-22:Q9GZX6 IL-21:Q9HBE4 IL-10:P22301 IL-13:P35225

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

GM-CSF<5pg/mL TNF-α<1pg/mL IL-2<3pg/mL IFN-γ<3pg/mL IL-9<5pg/mL IL-12p70<3pg/mL IL-4<1pg/mL IL-5<2pg/mL IL-6<5pg/mL IL-17A<3pg/mL IL-22<3pg/mL IL-21<5pg/mL IL-10<2pg/mL IL-13<5pg/mL

This kit is for detection of GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNF-α in human serum, plasma, biological fluid and cell culture supernatant.