Anti-Bmf (CT) Antibody

Rabbit Polyclonal Antibody specific to Bmf (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Bmf (CT) Rabbit Polyclonal Antibody

Gene Name

BMF

Gene ID

90427

NCBI Gene ID

UniProt

Q96LC9

Accession Number

NP_277038

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 171- 184 of human Bmf (accession no. NP_277038).

Target Antigen

Bcl-2-modifying factor

Target

Bmf (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

May play a role in apoptosis. Isoform 1 seems to be the main initiator.

Additionnal Information

Immunoblotting: use at 2.5-5ug/mL. A band of 25kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentration for their application. Positive control: HepG2 or 293 cell lysates.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes an epitope at the C-terminus of human Bmf (Bcl-2-Modifying Factor), a novel BH3-only protein. In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiaing the downstream apoptotic program. Bmf is constitutively expressed in many tissues.