Anti-TRIP230 Antibody

Mouse Monoclonal Antibody specific to TRIP230

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

TR-interacting protein 11, TRIP-11, Clonal evolution-related gene on chromosome 14 protein, Golgi-associated microtubule-binding protein 210, GMAP-210, Trip230

Gene Name

TRIP11

Gene ID

9321

NCBI Gene ID

UniProt

Q15643

Accession Number

NP_004230

Cellular Locus

Golgi apparatus, cis-Golgi network membrane, Cytoplasm, cytoskeleton, Endoplasmic reticulum-Golgi intermediate compartment membrane

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein corresponding to aa 1099-1372 of Trip230 expressed in E. coli.

Target Antigen

Thyroid receptor-interacting protein 11

Target

TRIP230

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Is a membrane tether required for vesicle tethering to Golgi. Has an essential role in the maintenance of Golgi structure and function (PubMed:25473115, PubMed:30728324). It is required for efficient anterograde and retrograde trafficking in the early secretory pathway, functioning at both the ER-to-Golgi intermediate compartment (ERGIC) and Golgi complex (PubMed:25717001). Binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. {PubMed:10189370, PubMed:25473115, PubMed:25717001, PubMed:30728324, PubMed:9256431}.

Additionnal Information

Immunoblotting, <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive control: MCF7, HBL100, and T24 cells and recombinant fusion protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human Trip230, a 230 kD protein. The gene for Trip230 is located on chromosome 14q31 in a region associated with genetic alterations and thyroid hormone response abnormalities. Trip230 interacts with Retinoblasoma Protein (Rb) and thyroid hormone receptor using two distinct domains.