Anti-Hu CD63 PE-Cy™5

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

HPB-ALL T cell line

Target Antigen

CD63

Clone

MEM-259

Applications

FC (QC tested)

Format

PE-Cy

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Heneberg P, Riegerová K, Kučera P: Pimecrolimus is a potent inhibitor of allergic reactions to hymenopteran venom extracts and birch pollen allergen in vitro. PLoS One. 2015 Nov 12;10 (11) :e0142953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=heneberg+p+riegerov%C3%A1+k+ku%C4%8Dera+p , *Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin-1 inhibits Ca (2+) -dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5 (9) :883-8. Erratum in: EMBO Rep. 2005 Sep;6 (9) :898. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15332114?dopt=

Other References

*Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels. J Biol Chem. 2008 Jan 22, URL: https://www.ncbi.nlm.nih.gov/pubmed/18211905?ordin, *Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung Cancer. 2007 Jul;57 (1) :46-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17350713?ordin, *Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb Haemost. 2005 Feb;93 (2) :311-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15711748?ordin, *Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation-linked T cell costimulatory element. J Immunol. 2004 Nov 15;173 (10) :6000-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15528334?ordin, *Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004 Aug 15;104 (4) :1183-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/15130945?ordin, *Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61 (1) :62-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15351990?ordin

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.