Anti-HLA-DR Alexa Fluor® 700

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Thymocyte membrane

Target Antigen

HLA-DR

Clone

MEM-12

Applications

FC (QC tested)

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28 (5) :288-97., URL: https://pubmed.ncbi.nlm.nih.gov/2435017/, *Sochorová K, Budínský V, Rožková D, Tobiášová Z, Dusilová-Sulková S, Spíšek R, Bartůňková J: Paricalcitol (19-nor-1,25-dihydroxyvitamin D2) and calcitriol (1,25-dihydroxyvitamin D3) exert potent immunomodulatory effects on dendritic cells and inhibit induction of antigen-specific T cells. Clin Immunol. 2009 Oct;133 (1) :69-77., URL: https://pubmed.ncbi.nlm.nih.gov/19660988/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages) .