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Anti-Hu IgM Alexa Fluor® 700

Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.

Product Specifications

Certification

RUO

Immunogen

Purified human IgM.

Target Antigen

IgM

Clone

CH2

Applications

FC (QC tested)

Concentration

0.1 mg/mL

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp, *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21 (6) :667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610?ordina, *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39 (2) :129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908?ordina, *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152 (2) :323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141?ordina

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody CH2 reacts with Fc fragment of human IgM.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

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