Anti-Hu CD8 Alexa Fluor® 700

The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Crude thymus membrane fraction.

Target Antigen

CD8

Clone

MEM-31

Applications

FC (QC tested)

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15 (4) :1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729 , *Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260 (FTO) . J Biomed Biotechnol. 2010;2010:841451., URL: https://www.ncbi.nlm.nih.gov/pubmed/20339516, *Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178 (7) :4402-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/17371997?itool, *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool, *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198 (10) :1453-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?itool, *Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74) . Folia Biol (Praha) . 1988;34 (1) :23-34., URL: https://pubmed.ncbi.nlm.nih.gov/2968928/, *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987) ., URL: , *Horejsi V et al.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol. (Praha) 32, 12 (1986) ., URL:

Other References

*Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177 (6) :3930-8., URL: https://pubmed.ncbi.nlm.nih.gov/16951356/, *Pang DJ, Hayday AC, Bijlmakers MJ: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282 (18) :13884-94., URL: https://pubmed.ncbi.nlm.nih.gov/17341584/, *van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249 (2) :395-408., URL: https://pubmed.ncbi.nlm.nih.gov/17869274/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa) . The antibody does not react with formaldehyde-fixed cells; negative in Western blotting application.