Anti-Hu CD177 Alexa Fluor® 647

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1) . CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN) .

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

Human granulocytes

Target Antigen

CD177

Clone

MEM-166

Applications

FC (QC tested), WB

Format

Alexa Fluor® 647

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb;81 (2) :458-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/17077162?ordin, *Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31) . J Biol Chem. 2007 Aug 10;282 (32) :23603-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/17580308?ordin, *Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar;90 (3) :405-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/15749675?ordin, *Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29;2 (1) :8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15050027?ordin, *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002) ., URL:

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.