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Anti-Phosphotyrosine Alexa Fluor® 647

Product Specifications

Certification

RUO

Reactivity

Species independent

Immunogen

Phosphotyrosine conjugated to bovine serum albumin.

Target Antigen

Phosphotyrosine

Clone

P-Tyr-01

Applications

WB, ICC, FC (QC tested)

Concentration

1 mg/mL

Format

Alexa Fluor® 647

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., URL: https://www.ncbi.nlm.nih.gov/pubmed/26650353 , *Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 (12) :1617-26., URL: http://www.ncbi.nlm.nih.gov/pubmed/12486104?itool=, *Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178 (4) :2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131?ordin, *Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191 (9) :1591-604., URL: https://pubmed.ncbi.nlm.nih.gov/10790433/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Applications Notes

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Isotype

Mouse IgG1

Frequently Asked Questions

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