Anti-Phosphotyrosine Alexa Fluor® 647
Product Specifications
Certification
RUO
Reactivity
Species independent
Immunogen
Phosphotyrosine conjugated to bovine serum albumin.
Target Antigen
Phosphotyrosine
Clone
P-Tyr-01
Applications
WB, ICC, FC (QC tested)
Concentration
1 mg/mL
Format
Alexa Fluor® 647
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., URL: https://www.ncbi.nlm.nih.gov/pubmed/26650353 , *Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 (12) :1617-26., URL: http://www.ncbi.nlm.nih.gov/pubmed/12486104?itool=, *Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178 (4) :2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131?ordin, *Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191 (9) :1591-604., URL: https://pubmed.ncbi.nlm.nih.gov/10790433/
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Isotype
Mouse IgG1
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