Anti-Ki-67 Alexa Fluor® 647
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Product Specifications
Certification
RUO
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Target Antigen
Ki-67
Clone
Ki-67
Applications
FC (QC tested), WB, ICC
Format
Alexa Fluor® 647
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Applications Notes
6
Species
Cow, Human
CAS Number
9007-83-4
Isotype
Mouse IgG1
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