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Anti-Ki-67 Alexa Fluor® 647

Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.

Product Specifications

Certification

RUO

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Target Antigen

Ki-67

Clone

Ki-67

Applications

FC (QC tested), WB, ICC

Format

Alexa Fluor® 647

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123 (3) :513-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/8227122 , *Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz) . 1995;43 (2) :117-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/8744726, *Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133 (4) :1710-5., URL: https://pubmed.ncbi.nlm.nih.gov/6206131/, *Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1 (3) :199-206., URL: https://www.ncbi.nlm.nih.gov/pubmed/2103495, *Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31 (1) :13-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/6339421, *Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov;185 (1) :50-9., URL: https://pubmed.ncbi.nlm.nih.gov/2680541/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Applications Notes

6

Species

Cow, Human

CAS Number

9007-83-4

Isotype

Mouse IgG1

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