Anti-HLA-ABCE Alexa Fluor® 488

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

IFN-gamma-treated human melanoma cells Colo 38

Target Antigen

HLA-ABCE

Clone

TP25.99SF

Applications

FC (QC tested), WB

Concentration

0.1 mg/mL

Format

Alexa Fluor® 488

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148 (10) :3202-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=tanabe+m+sekimata+m+ferrone+s , *Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000 Sep 15;165 (6) :3275-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/10975844 , *Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F: Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol. 2003 Aug 15;171 (4) :1918-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902494 , *Moy FJ, Desai SA, Wang X, Noronha EJ, Zhou Q, Ferrone S, Powers R: Analysis by NMR spectroscopy of the structural homology between the linear and the cyclic peptide recognized by anti-human leukocyte antigen class I monoclonal antibody TP25.99. J Biol Chem. 2000 Aug 11;275 (32) :24679-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/10825172 , *Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61 (11) :1074-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=blaschitz+a+hutter+h+leitner+v , *Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61 (11) :1177-95., URL: https://pubmed.ncbi.nlm.nih.gov/11137224/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.