Anti-FoxP3 Alexa Fluor® 488

FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.

Product Specifications

Certification

RUO

Reactivity

Human, Mouse

Immunogen

Full-length His-tagged recombinant murine FoxP3

Target Antigen

FoxP3

Clone

3G3

Applications

FC (QC tested), WB

Concentration

0.5 mg/mL

Format

Alexa Fluor® 488

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75 (12) :1040-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/19845018?itool, *Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103 (17) :6659-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16617117?itool

Other References

*Kuhn A, Beissert S, Krammer PH: CD4 (+) CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301 (1) :71-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/18985367?itool, *Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114 (18) :3727-35. , URL: https://www.ncbi.nlm.nih.gov/pubmed/19641188, *Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31 (3) :401-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/19766083?itool, *Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21 (6) :612-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/19854631?itool, *Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18802345?itool

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.