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Anti-Bov CD9 Alexa Fluor® 488

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Product Specifications

Certification

RUO

Reactivity

Human, Cow

Immunogen

Bovine thrombocytes.

Target Antigen

CD9

Clone

IVA50

Applications

FC (QC tested)

Concentration

0.5 mg/mL

Format

Alexa Fluor® 488

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Sopp P, Howard CJ: Investigation of PC36 (BoCD45R) . Vet Immunol Immunopathol. 1996 Aug;52 (4) :271-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896215?dopt=A, *Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996), URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204?dopt=A, *Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18 (8) :365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076?dopt=A, *Tomaskova J, Dusinsky R, Horovska L, Simon M: A set of monoclonal antibodies specific for bovine cell surface molecule CD9. Folia Biol (Praha) . 1999;45 (5) :225-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/10730891?dopt=, *Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52 (4) :363-70. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8896226?dopt=A

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.

Isotype

Mouse IgG2

Frequently Asked Questions

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