Anti-Hu CD45 Alexa Fluor® 488

*Wellhausen SR, Slone SP, Miller JJ: Clone-specific anti-CD45 blocking factor in patient plasma. Cytometry B Clin Cytom. 2007 Sep;72 (5) :423-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17311353, *Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4 (+) -T-cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin Diagn Lab Immunol. 2002 Sep;9 (5) :1085-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/12204964, *Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti-CCR4 monoclonal antibody exercises potent ADCC-mediated antitumor effect in the novel tumor-bearing humanized NOD/Shi-scid, IL-2Rgamma (null) mouse model. Cancer Immunol Immunother. 2009 Aug;58 (8) :1195-206., URL: https://pubmed.ncbi.nlm.nih.gov/19048251/, *Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS-induced arachidonic acid metabolism in macrophages. Biochim Biophys Acta. 2000 Feb 28;1495 (3) :212-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10699460, *Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Hematol. 2010 Feb;32 (1 Pt 1) :e40-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/20088999, *Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS One. 2013;8 (3) :e58565., URL: https://www.ncbi.nlm.nih.gov/pubmed/23555586, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353

*Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105 (8) :3295-302. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin, *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16 (4) :256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594?ordina, *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176 (6) :3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710?ordina, *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44 (15) :3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482?ordina, *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. , URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742?ordina

The mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.