Anti-Rt PLSCR1 Ascites

PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.

Product Specifications

Certification

RUO

Reactivity

Rat

Immunogen

Pooled lipid raft fraction isolated from RBL-2H3 cells

Target Antigen

PLSCR1

Clone

13A6 [TEC-23]

Applications

IP, WB, ICC, FC (QC tested)

Format

Ascites

Buffer

Stabilized with 15mM sodium azide.

References & Citations

*Smrž D, Lebduška P, Dráberová L, Korb J, Dráber P: Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem. 2008 Apr 18;283 (16) :10904-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/18281686?ordin

Other References

*Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276 (23) :20407-12. Epub 2001 Mar 20., URL: https://pubmed.ncbi.nlm.nih.gov/11259432/, *Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62 (2) :397-402. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11809687?dopt=, *Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78 (17) :8983-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/15308695?dopt=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.

Applications Notes

Western blotting: Recommended dilution: 1 μg/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.Flow cytometry: Intracellular staining.