Anti-HLA-E PE

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells) .

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Recombinant human HLA-E

Target Antigen

HLA-E

Clone

3D12

Applications

FC (QC tested)

Concentration

0.1 mg/mL

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13 (9) :822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c , *Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains. Eur J Immunol. 2015 Aug;45 (8) :2356-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/25982269 , *Ravindranath MH, Pham T, El-Awar N, Kaneku H, Terasaki PI: Anti-HLA-E mAb 3D12 mimics MEM-E/02 in binding to HLA-B and HLA-C alleles: Web-tools validate the immunogenic epitopes of HLA-E recognized by the antibodies. Mol Immunol. 2011 Jan;48 (4) :423-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/21145594 , *Sensi M, Pietra G, Molla A, Nicolini G, Vegetti C, Bersani I, Millo E, Weiss E, Moretta L, Mingari MC, Anichini A: Peptides with dual binding specificity for HLA-A2 and HLA-E are encoded by alternatively spliced isoforms of the antioxidant enzyme peroxiredoxin 5. Int Immunol. 2009 Mar;21 (3) :257-68., URL: https://pubmed.ncbi.nlm.nih.gov/19181932/, *McMurtrey C, Harriff MJ, Swarbrick GM, Duncan A, Cansler M, Null M, Bardet W, Jackson KW, Lewinsohn DA, Hildebrand W, Lewinsohn DM: T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells. PLoS One. 2017 Nov 27;12 (11) :e0188288., URL: https://www.ncbi.nlm.nih.gov/pubmed/29176828 , *Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE: HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998 Apr 28;95 (9) :5199-204., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=lee+n+llano+m+carretero+m

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 3D12 (also known as 3D12HLA-E) recognizes native extracellular part of HLA-E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA-E.