What is the catalog number for Anti-MICA/MICB PE?

The catalog number for Anti-MICA/MICB PE is 1P-822-C100.

What sizes are available for Anti-MICA/MICB PE?

Anti-MICA/MICB PE is available in the following size(s): 0.1 mg.

Is Anti-MICA/MICB PE in stock?

Anti-MICA/MICB PE is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Anti-MICA/MICB PE?

Anti-MICA/MICB PE is manufactured by AFG-XBO.

What is the estimated delivery time for Anti-MICA/MICB PE?

Estimated delivery time for Anti-MICA/MICB PE is approximately 10 business day(s), depending on your location.

Anti-MICA/MICB PE

MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Transfected C1R cells expressing MICA

Target Antigen

MICA/MICB

Clone

6D4

Applications

FC (QC tested)

Concentration

0.1 mg/mL

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Ghadially H, Brown L, Lloyd C, Lewis L, Lewis A, Dillon J, Sainson R, Jovanovic J, Tigue NJ, Bannister D, Bamber L, Valge-Archer V, Wilkinson RW: MHC class I chain-related protein A and B (MICA and MICB) are predominantly expressed intracellularly in tumour and normal tissue. Br J Cancer. 2017 Apr 25;116 (9) :1208-1217., URL: https://pubmed.ncbi.nlm.nih.gov/28334733/, *Ritter C, Fan K, Paulson KG, Nghiem P, Schrama D, Becker JC: Reversal of epigenetic silencing of MHC class I chain-related protein A and B improves immune recognition of Merkel cell carcinoma. Sci Rep. 2016 Feb 23;6:21678. doi: 10.1038/srep21678., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=ritter+c%2C+fan+k%2C+paulson+kg , *Del Toro-Arreola S, Arreygue-Garcia N, Aguilar-Lemarroy A, Cid-Arregui A, Jimenez-Perez M, Haramati J, Barros-Nuñ ez P, Gonzalez-Ramella O, Del Toro-Arreola A, Ortiz-Lazareno P, Hernandez-Flores G, Bravo-Cuellar A, Daneri-Navarro A, Jave-Suarez LF: MHC class I-related chain A and B ligands are differentially expressed in human cervical cancer cell lines. Cancer Cell Int. 2011 Jun 1;11:15. doi: 10.1186/1475-2867-11-15., URL: https://pubmed.ncbi.nlm.nih.gov/21631944/, *Carreno BM, Garbow JR, Kolar GR, Jackson EN, Engelbach JA, Becker-Hapak M, Carayannopoulos LN, Piwnica-Worms D, Linette GP: Immunodeficient mouse strains display marked variability in growth of human melanoma lung metastases. Clin Cancer Res. 2009 May 15;15 (10) :3277-86., URL: https://pubmed.ncbi.nlm.nih.gov/19447870/, *Sester M, Koebernick K, Owen D, Ao M, Bromberg Y, May E, Stock E, Andrews L, Groh V, Spies T, Steinle A, Menz B, Burgert HG: Conserved amino acids within the adenovirus 2 E3/19K protein differentially affect downregulation of MHC class I and MICA/B proteins. J Immunol. 2010 Jan 1;184 (1) :255-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/19949079 , *Xu X, Rao G, Gaffud MJ, Ding HG, Maki G, Klingemann HG, Groh V, Spies T, Caillat-Zucman S, Gattuso P, Plate J, Prinz RA: Clinicopathological significance of major histocompatibility complex class I-related chain a and B expression in thyroid cancer. J Clin Endocrinol Metab. 2006 Jul;91 (7) :2704-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/16621903 , *Steinle A, Li P, Morris DL, Groh V, Lanier LL, Strong RK, Spies T: Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family. Immunogenetics. 2001 May-Jun;53 (4) :279-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/11491531 , *Groh V, Rhinehart R, Secrist H, Bauer S, Grabstein KH, Spies T: Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci U S A. 1999 Jun 8;96 (12) :6879-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=broad+tumor-associated+expression+and+recognition+by+tumor-derived , *Groh V, Bahram S, Bauer S, Herman A, Beauchamp M, Spies T: Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium. Proc Natl Acad Sci U S A. 1996 Oct 29;93 (22) :12445-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8901601

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of CD314 (NKG2D), and is able to block CD314-mediated activation of NK cells and cytotoxic T cells.