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Anti-N-sulfated heparan sulfate PE

Heparan sulfate (HS) proteoglycans are expressed on cell surfaces and in the extracellular matrix, and take part in developmental, regenerative, as well as pathological processes. By interaction with extracellular matrix components, growth factors, enzymes, and their inhibitors, they regulate and influence tissue distribution of the cells and biological activities of the proteins. N-sulfated heparan sulfate proteoglycans are located in specific microdomains in the plasma membrane, independent on those formed around N-acetyl-rich heparan sulfate, and play different role in the cell signaling.

Product Specifications

Certification

RUO

Immunogen

MethA murine fibrosarcoma

Target Antigen

N-sulfated heparan sulfate

Clone

HepSS-1

Applications

FC (QC tested)

Concentration

0.1 mg/mL

Format

PE

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kure S, Yoshie O: A syngeneic monoclonal antibody to murine Meth-A sarcoma (HepSS-1) recognizes heparan sulfate glycosaminoglycan (HS-GAG) : cell density and transformation dependent alteration in cell surface HS-GAG defined by HepSS-1. J Immunol. 1986 Dec 15;137 (12) :3900-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/2431047, *van den Born J, Salmivirta K, Henttinen T, Ostman N, Ishimaru T, Miyaura S, Yoshida K, Salmivirta M: Novel heparan sulfate structures revealed by monoclonal antibodies. J Biol Chem. 2005 May 27;280 (21) :20516-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/15778504, *Lipscombe RJ, Nakhoul AM, Sanderson CJ, Coombe DR: Interleukin-5 binds to heparin/heparan sulfate. A model for an interaction with extracellular matrix. J Leukoc Biol. 1998 Mar;63 (3) :342-50., URL: https://pubmed.ncbi.nlm.nih.gov/9500522/, *Crim RL, Audet SA, Feldman SA, Mostowski HS, Beeler JA: Identification of linear heparin-binding peptides derived from human respiratory syncytial virus fusion glycoprotein that inhibit infectivity. J Virol. 2007 Jan;81 (1) :261-71. Epub 2006 Oct 18., URL: https://www.ncbi.nlm.nih.gov/pubmed/17050595, *Chung JS, Shiue LH, Duvic M, Pandya A, Cruz PD Jr, Ariizumi K: Sézary syndrome cells overexpress syndecan-4 bearing distinct heparan sulfate moieties that suppress T-cell activation by binding DC-HIL and trapping TGF-beta on the cell surface. Blood. 2011 Mar 24;117 (12) :3382-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/21252093, *Kure S, Yoshie O, Aso H: Metastatic potential of murine B16 melanoma correlates with reduced surface heparan sulfate glycosaminoglycan. Jpn J Cancer Res. 1987 Nov;78 (11) :1238-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/2961717, *Mii Y, Yamamoto T, Takada R, Mizumoto S, Matsuyama M, Yamada S, Takada S, Taira M: Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in Xenopus. Nat Commun. 2017 Dec 7;8 (1) :1973., URL: https://pubmed.ncbi.nlm.nih.gov/29215008/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody HepSS-1 (also known as HepSS1) recognizes N-sulfated heparan sulfate (extracellular antigen) present in many species.

Applications Notes

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Species

Species independent

CAS Number

9007-83-4

Isotype

Mouse IgM

Curated Selection

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