Anti-DR3 PE

DR3, also known as APO-3, TRAMP or TNFRSF25, is a death domain-containing receptor of TNFR family, which is expressed preferentially in peripheral blood leukocytes and in the lymphocyte-enriched tissues. Its expression has been shown to be especially up-regulated in activated T cells. DR3 participates e.g. in the removal of self-reactive T cells in the thymus. The ligand for DR3 is TL1A (TNF-like ligand 1A), which is expressed in a variety of cell types (induced by inflammatory stimuli), and can also be released as a soluble factor. The TL1A/DR3 axis has been shown to costimulate T cells to produce a wide variety of cytokines and leads to T cell differentiation towards Th1 and Th17 types.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human DR3-Ig fusion protein

Target Antigen

DR3

Clone

JD3

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Nakayama M, Ishidoh K, Kayagaki N, Kojima Y, Yamaguchi N, Nakano H, Kominami E, Okumura K, Yagita H: Multiple pathways of TWEAK-induced cell death. J Immunol. 2002 Jan 15;168 (2) :734-43., URL: http://www.ncbi.nlm.nih.gov/pubmed/11777967 , *Cavallini C, Lovato O, Bertolaso A, Pacelli L, Zoratti E, Zanolin E, Krampera M, Zamò A, Tecchio C, Cassatella MA, Pizzolo G, Scupoli MT: The TNF-family cytokine TL1A inhibits proliferation of human activated B cells. PLoS One. 2013;8 (4) :e60136., URL: http://www.ncbi.nlm.nih.gov/pubmed/23565196 , *Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA: Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J. 2011 Jan;25 (1) :409-19., URL: http://www.ncbi.nlm.nih.gov/pubmed/20826539 , *Yi B, Zhang M, Schwartz-Albiez R, Cao Y: Mechanisms of the apoptosis induced by CD176 antibody in human leukemic cells. Int J Oncol. 2011 Jun;38 (6) :1565-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/21455576

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody JD3 recognizes an extracellular epitope of DR3 (APO-3, TNFRSF25), a transmembrane protein of TNFR superfamily expressed mainly in lymphocyte-enriched tissues.