Anti-Hu CD88 PE

CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.

Product Specifications

Certification

RUO

Reactivity

Rabbit, Ferret, Cow, Human

Immunogen

Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR

Target Antigen

CD88

Clone

S5/1

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kiener HP, Baghestanian M, Dominkus M, Walchshofer S, Ghannadan M, Willheim M, Sillaber C, Graninger WB, Smolen JS, Valent P: Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis. Arthritis Rheum. 1998 Feb;41 (2) :233-45., URL: http://www.ncbi.nlm.nih.gov/pubmed/9485081, *Conway Morris A, Kefala K, Wilkinson TS, Dhaliwal K, Farrell L, Walsh T, Mackenzie SJ, Reid H, Davidson DJ, Haslett C, Rossi AG, Sallenave JM, Simpson AJ: C5a mediates peripheral blood neutrophil dysfunction in critically ill patients. Am J Respir Crit Care Med. 2009 Jul 1;180 (1) :19-28., URL: http://www.ncbi.nlm.nih.gov/pubmed/19324972 , *Cain SA, Monk PN: The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg (74) . J Biol Chem. 2002 Mar 1;277 (9) :7165-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/11773063 , *Camous L, Roumenina L, Bigot S, Brachemi S, Frémeaux-Bacchi V, Lesavre P, Halbwachs-Mecarelli L: Complement alternative pathway acts as a positive feedback amplification of neutrophil activation. Blood. 2011 Jan 27;117 (4) :1340-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=camous+l+roumenina+l