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Anti-Hu CD94 PE

CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.

Product Specifications

Certification

RUO

Immunogen

Cultured human NK cells

Target Antigen

CD94

Clone

HP-3D9

Applications

FC (QC tested)

Format

PE

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA: CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets. Blood. 2010 Jan 14;115 (2) :274-81, URL: http://www.ncbi.nlm.nih.gov/pubmed/19897577 , *Phillips JH, Chang C, Mattson J, Gumperz JE, Parham P, Lanier LL: CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes. Immunity. 1996 Aug;5 (2) :163-72., URL: http://www.ncbi.nlm.nih.gov/pubmed/8769479 , *Seo N, Tokura Y, Ishihara S, Takeoka Y, Tagawa S, Takigawa M: Disordered expression of inhibitory receptors on the NK1-type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites. Clin Exp Immunol. 2000 Jun;120 (3) :413-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10844517 , *Hallermalm K, Seki K, De Geer A, Motyka B, Bleackley RC, Jager MJ, Froelich CJ, Kiessling R, Levitsky V, Levitskaya J: Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes. J Immunol. 2008 Mar 15;180 (6) :3766-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/18322182 , *Bovenschen HJ, Van De Kerkhof PC, Gerritsen WJ, Seyger MM: The role of lesional T cells in recalcitrant psoriasis during infliximab therapy. Eur J Dermatol. 2005 Nov-Dec;15 (6) :454-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16280298 , *Ntrivalas EI, Kwak-Kim JY, Gilman-Sachs A, Chung-Bang H, Ng SC, Beaman KD, Mantouvalos HP, Beer AE: Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology. Hum Reprod. 2001 May;16 (5) :855-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11331628 , *Romero P, Ortega C, Palma A, Molina IJ, Peñ a J, Santamaría M: Expression of CD94 and NKG2 molecules on human CD4 (+) T cells in response to CD3-mediated stimulation. J Leukoc Biol. 2001 Aug;70 (2) :219-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/11493613, *Wada H, Matsumoto N, Maenaka K, Suzuki K, Yamamoto K: The inhibitory NK cell receptor CD94/NKG2A and the activating receptor CD94/NKG2C bind the top of HLA-E through mostly shared but partly distinct sets of HLA-E residues. Eur J Immunol. 2004 Jan;34 (1) :81-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/14971033

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70 kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.

Applications Notes

6

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1 kappa

Curated Selection

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