Anti-Hu CD19 PE
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Product Specifications
Certification
RUO
Immunogen
Human CCL (chronic lymphocytic leukemia) cells
Target Antigen
CD19
Clone
4G7
Applications
FC (QC tested)
Format
PE
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Muench MO, Roncarolo MG, Namikawa R: Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood. 1997 Feb 15;89 (4) :1364-75., URL: http://www.ncbi.nlm.nih.gov/pubmed/9028960, *Stockmeyer B, Dechant M, van Egmond M, Tutt AL, Sundarapandiyan K, Graziano RF, Repp R, Kalden JR, Gramatzki M, Glennie MJ, van de Winkel JG, Valerius T: Triggering Fc alpha-receptor I (CD89) recruits neutrophils as effector cells for CD20-directed antibody therapy. J Immunol. 2000 Nov 15;165 (10) :5954-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11067958 , *Dubois B, Massacrier C, Caux C: Selective attraction of naive and memory B cells by dendritic cells. J Leukoc Biol. 2001 Oct;70 (4) :633-41. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11590201 , *Basu S, Lynne CM, Ruiz P, Aballa TC, Ferrell SM, Brackett NL: Cytofluorographic identification of activated T-cell subpopulations in the semen of men with spinal cord injuries. J Androl. 2002 Jul-Aug;23 (4) :551-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/12065463 , *Köller M, Zwölfer B, Steiner G, Smolen JS, Scheinecker C: Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int Immunol. 2004 Nov;16 (11) :1595-604. , URL: http://www.ncbi.nlm.nih.gov/pubmed/15381672 , *Treusch M, Vonthein R, Baur M, Günaydin I, Koch S, Stübiger N, Eckstein AK, Peter HH, Ness T, Zierhut M, Kötter I: Influence of human recombinant interferon-alpha2a (rhIFN-alpha2a) on altered lymphocyte subpopulations and monocytes in Behcet's disease. Rheumatology (Oxford) . 2004 Oct;43 (10) :1275-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/15252211 , *Porcellini S, Vallanti G, Nozza S, Poli G, Lazzarin A, Tambussi G, Siccardi AG, Grassi F: Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL-2 administration. AIDS. 2003 Jul 25;17 (11) :1621-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/12853744 , *Andersen P, Pedersen MW, Woetmann A, Villingshø j M, Stockhausen MT, Odum N, Poulsen HS: EGFR induces expression of IRF-1 via STAT1 and STAT3 activation leading to growth arrest of human cancer cells. Int J Cancer. 2008 Jan 15;122 (2) :342-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17918184 , *Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R, Pession A: Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t (9;11) (p22; q23) . Oncol Rep. 2006 Jan;15 (1) :207-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/16328057 , *Leukocyte Typing II., Reinherz E.L. et al. (Eds.), Springer-Verlag (1985) ., URL: , *Mueller GA, Smith AM, Chapman MD, Rule GS, Benjamin DC: Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem. 2001 Mar 23;276 (12) :9359-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/11134039, *Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD: The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol. 2001 Jun;107 (6) :977-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/11398074
Other References
*Shi X, Xie C, Chang S, Zhou XJ, Tedder T, Mohan C: CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis. Arthritis Rheum. 2007 Sep;56 (9) :3057-69., URL: https://pubmed.ncbi.nlm.nih.gov/17763445/, *van Zelm MC, Reisli I, van der Burg M, Castaño D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher B, Patiño PJ, van Dongen JJ, Franco JL: An antibody-deficiency syndrome due to mutations in the CD19 gene. N Engl J Med. 2006 May 4;354 (18) :1901-12., URL: https://pubmed.ncbi.nlm.nih.gov/16672701/, *Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99 (2) :584-9., URL: https://pubmed.ncbi.nlm.nih.gov/11781242/, *Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF: CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999 Jun 15;162 (12) :7088-94., URL: https://pubmed.ncbi.nlm.nih.gov/10358152/
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The mouse monoclonal antibody 4G7 recognizes an extracellular epitope of human CD19.
Applications Notes
6
Species
Human
CAS Number
9007-83-4
Isotype
Mouse IgG1 kappa
Curated Selection
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