Anti-Hu STRO-1 PE

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.

Product Specifications

Certification

RUO

Reactivity

Human, Rabbit, Rat

Immunogen

Human CD34 positive bone marrow cells

Target Antigen

STRO-1

Clone

STRO-1

Applications

FC (QC tested)

Concentration

0.1 mg/mL

Format

Buffer

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody. J Bone Miner Res. 1999 Mar;14 (3) :351-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/10027900, *Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004 May 1;103 (9) :3313-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/14715641, *Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy. J Electron Microsc (Tokyo) . 2009 Dec;58 (6) :363-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/19525367, *Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001 Jun;16 (6) :1120-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11393789, *Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003 May 1;116 (Pt 9) :1827-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/12665563, *Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005 Oct;84 (10) :907-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/16183789, *Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood. 1991 Jul 1;78 (1) :55-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/2070060, *Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 2012;21 (10) :2201-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/22469435, *Fitter S, Gronthos S, Ooi SS, Zannettino AC: The mesenchymal precursor cell marker antibody STRO-1 binds to cell surface heat shock cognate 70. Stem Cells 2017 Apr;35 (4) :940-951., URL: https://pubmed.ncbi.nlm.nih.gov/28026090/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.