Anti-Ms CD25 PE

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R) . Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

B6.1 CTL cell line

Target Antigen

CD25

Clone

PC61.5

Applications

FC (QC tested)

Concentration

0.5 mg/mL

Format

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078., URL: http://www.ncbi.nlm.nih.gov/pubmed/20037737 , *Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181 (10) :7221-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/18981144 , *Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22 (13) :4556-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/12052865, *Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89 (11) :4005-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/9166839, *Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD (4+) CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78 (3) :725-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16000396, *Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181 (8) :5204-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18832672, *Kotner J, Tarleton R: Endogenous CD4 (+) CD25 (+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75 (2) :861-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17101658, *Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74 (5) :2414-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10666272