Anti-NTAL PE

NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Product Specifications

Certification

RUO

Reactivity

Human, Mouse

Immunogen

Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Target Antigen

NTAL

Clone

NAP-07

Applications

FC-IC (QC tested)

Concentration

0.1 mg/mL

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Lebduška P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328 (1-2) :139-51., URL: https://pubmed.ncbi.nlm.nih.gov/17900607/, *Volná P, Lebduška P, Dráberová L, Šímová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Hořejší V, Malissen M, Dráber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200 (8) :1001-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/15477348?dopt=, *Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196 (12) :1617-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12486104?dopt=

Other References

*Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4 (8) :603-16., URL: https://pubmed.ncbi.nlm.nih.gov/15286727/, *Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27 (12) :4406-15., URL: https://pubmed.ncbi.nlm.nih.gov/17420272/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.

Applications Notes

Flow cytometry: Intracellular staining; recommended dilution: 1-5 μg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.