Anti-Hu CD114 PE

CD114 / G-CSFR (granulocyte colony-stimulating factor receptor, also known as CSF3R) is a type I transmembrane glycoprotein which upon binding of its ligand (G-CSF, granulocyte colony-stimulating factor) homodimerizes and activates signaling transduction to mediate cell proliferation, survival, and differentiation. It is expressed by granulocytes at all stages of their differentiation, as well as by monocytes, dendritic cells, and mature platelets. Among non-hematopoietic cells, it is expressed e.g. by endothelial cells, placenta, trophoblasts, and many tumor cell lines. This antigen is a target for stem cell mobilization for blood stem cell transplantation, for enhancing recovery of myelopoiesis following chemotherapy and in the treatment of patients with severe chronic neutropenia.

Product Specifications

Certification

RUO

Negative Species

Mouse

Reactivity

Human

Immunogen

CHO cells transfected with human CD114

Target Antigen

CD114

Clone

LMM741

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Layton JE, Shimamoto G, Osslund T, Hammacher A, Smith DK, Treutlein HR, Boone T: Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor. J Biol Chem. 1999 Jun 18;274 (25) :17445-51., URL: https://pubmed.ncbi.nlm.nih.gov/10364174/, *Layton JE, Hall NE, Connell F, Venhorst J, Treutlein HR: Identification of ligand-binding site III on the immunoglobulin-like domain of the granulocyte colony-stimulating factor receptor. J Biol Chem. 2001 Sep 28;276 (39) :36779-87., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=layton+je+hall+ne+connel+f , *Gibbs KD Jr, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R: Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments. Blood. 2011 Apr 21;117 (16) :4226-33. doi: 10.1182/blood-2010-07-298232., URL: https://pubmed.ncbi.nlm.nih.gov/21357764/, *Tchou J, Zhang PJ, Bi Y, Satija C, Marjumdar R, Stephen TL, Lo A, Chen H, Mies C, June CH, Conejo-Garcia J, Puré E: Fibroblast activation protein expression by stromal cells and tumor-associated macrophages in human breast cancer. Hum Pathol. 2013 Nov;44 (11) :2549-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/24074532 , *Cimato TR, Palka BA, Lang JK, Young RF: LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis. PLoS One. 2013 Aug 26;8 (8) :e73861., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=cimato+tr+palka+ba+lang+ba , *Layton JE, Iaria J, Smith DK, Treutlein HR: Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis. J Biol Chem. 1997 Nov 21;272 (47) :29735-41., URL: http://www.ncbi.nlm.nih.gov/pubmed/9368043

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody LMM741 recognizes an extracellular epitope of CD114 (colony stimulating factor 3 receptor), a 130 kDa transmembrane glycoprotein expressed on granulocytes and their differentiation stages, on monocytes, platelets, endothelial cells and placenta. It is absent from lymphocytes and erythrocytes.