Anti-Hu CD103 FITC

CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain) . It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

HTLV-1 induced human T cell line MAPS16

Target Antigen

CD103

Clone

Ber-ACT8

Applications

FC (QC tested)

Format

FITC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct;77 (19) :10456-67., URL: http://www.ncbi.nlm.nih.gov/pubmed/12970431 , *Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1;205 (9) :2139-49., URL: https://www.ncbi.nlm.nih.gov/pubmed/18710932, *Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15;183 (10) :6346-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/19841178, *Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul;84 (1) :311-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18451325, *Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T-cell involvement in humoral immunity. Blood. 2003 Nov 15;102 (10) :3693-701., URL: https://www.ncbi.nlm.nih.gov/pubmed/12881309, *Kost CB, Holden JT, Mann KP: Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep;74 (5) :282-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/18500740, *French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy? Br J Cancer. 2002 Oct 21;87 (9) :1034-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/12434297, *Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul;13 (7) :426-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/15217363, *León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads. Lab Invest. 2004 Jun;84 (6) :804-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/15154017

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.