Anti-Hu BCL2 FITC

Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.

Product Specifications

Certification

RUO

Negative Species

Mouse

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Target Antigen

BCL2

Clone

Bcl-2/100

Applications

FC-IC (QC tested)

Format

FITC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33 (1) :67-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/19499887, *Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4 (+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct;81 (20) :11426-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/17670831, *Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21 (2) :225-31., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=laflamme%20c%20cormier%20y , *Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19 (12) :4828-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/10366617 , *Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13 (7) :1235-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16485035

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.