Anti-Ms CD54 FITC

CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

The rat monoclonal antibody YN1/1.7.4 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells the expression of CD54 is upregulated by activation.

*Sasaki M, Elrod JW, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: CYP450 dietary inhibitors attenuate TNF-alpha-stimulated endothelial molecule expression and leukocyte adhesion. Am J Physiol Cell Physiol. 2004 Apr286(4):C931-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15001427?itool,*Salas A, Gironella M, Salas A, Soriano A, Sans M, Iovanna J, Piqué JM, Panés J: Nitric oxide supplementation ameliorates dextran sulfate sodium-induced colitis in mice. Lab Invest. 2002 May82(5):597-607., URL: https://www.ncbi.nlm.nih.gov/pubmed/12004000?itool,*Chin JE, Winterrowd GE, Hatfield CA, Brashler JR, Griffin RL, Vonderfecht SL, Kolbasa KP, Fidler SF, Shull KL, Krzesicki RF, Ready KA, Dunn CJ, Sly LM, Staite ND, Richards IM: Involvement of intercellular adhesion molecule-1 in the antigen-induced infiltration of eosinophils and lymphocytes into the airways in a murine model of pulmonary inflammation. Am J Respir Cell Mol Biol. 1998 Feb18(2):158-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/9476902?itool=,*Greicius G, Westerberg L, Davey EJ, Buentke E, Scheynius A, Thyberg J, Severinson E: Microvilli structures on B lymphocytes: inducible functional domains? Int Immunol. 2004 Feb16(2):353-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/14734621?itool,*Ma Z, Sharp KA, Janmey PA, Finkel TH: Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity. PLoS Biol. 2008 Feb6(2):e43., URL: https://www.ncbi.nlm.nih.gov/pubmed/18303949?itool,*Sasaki M, Ostanin D, Elrod JW, Oshima T, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: TNF-alpha -induced endothelial cell adhesion molecule expression is cytochrome P-450 monooxygenase dependent. Am J Physiol Cell Physiol. 2003 Feb284(2):C422-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12388057?itool,*Horley KJ, Carpenito C, Baker B, Takei F: Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 1989 Oct8(10):2889-96., URL: https://www.ncbi.nlm.nih.gov/pubmed/2573511?itool=,*Burns AR, Takei F, Doerschuk CM: Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol. 1994 Oct 1153(7):3189-98., URL: https://www.ncbi.nlm.nih.gov/pubmed/7916369?itool=,*Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J: VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest. 2000 Oct80(10):1541-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/11045571?itool,*Barrett SP, Riordon A, Toh BH, Gleeson PA, van Driel IR: Homing and adhesion molecules in autoimmune gastritis. J Leukoc Biol. 2000 Feb67(2):169-73, URL: https://www.ncbi.nlm.nih.gov/pubmed/10670576?itool,