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Anti-Ms CD4 FITC

CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type) . Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1) ; HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1) ; IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120) . CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency) .

Product Specifications

Certification

RUO

Immunogen

Mouse CTL clone V4 cells

Target Antigen

CD4

Clone

GK1.5

Applications

FC (QC tested)

Concentration

0.5 mg/mL

Format

FITC

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85 (8) :1167-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/18431238?ordin, *Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180 (10) :6593-603., URL: https://www.ncbi.nlm.nih.gov/pubmed/18453578?ordin, *Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8 (4) :388-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/17322886?ordin, *Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184 (3) :1083-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/9064325?ordina, *Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10 (6) :587-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/8003337?ordina, *Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152 (6) :2729-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/7511624?ordina, *Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174 (6) :1617-27., URL: https://www.ncbi.nlm.nih.gov/pubmed/1683894?ordina, *Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131 (5) :2178-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/6195255?ordina, *Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131 (5) :2445-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/6415170?ordina, *Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al.: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/6195085?ordina

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa) .

Applications Notes

Flow cytometry: Recommended dilution: 1-2 μg/ml.

Species

Mouse

CAS Number

9007-83-4

Isotype

Rat IgG2b

Curated Selection

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