Anti-Hu TCR alpha/beta Biotin

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Product Specifications

Certification

RUO

Reactivity

Human

Target Antigen

TCR alpha/beta

Clone

IP26

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113 (2) :358-69., URL: http://www.ncbi.nlm.nih.gov/pubmed/18922855 Sulliv

Other References

*von Boehmer H: T cell development and selection in the thymus. Bone Marrow Transplant. 1992;9 Suppl 1:46-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/1324045?ordina, *Levelt CN, Wang B, Ehrfeld A, Terhorst C, Eichmann K: Regulation of T cell receptor (TCR) -beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex. Eur J Immunol. 1995 May;25 (5) :1257-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/7774628?ordina, *Michie AM, Zúñiga-Pflücker JC: Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Semin Immunol. 2002 Oct;14 (5) :311-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/12220932?ordin, *Croxford AL, Akilli-Ozturk O, Rieux-Laucat F, Förster I, Waisman A, Buch T: MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor. Eur J Immunol. 2008 Feb;38 (2) :391-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18203137?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.