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Anti-Hu CD82 Biotin

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

C91/PL (human HTLV-1+ T cell line)

Target Antigen

CD82

Clone

C33

Applications

FC (QC tested), IP, WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8 (395) :rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269 , *Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149 (5) :1435-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/8909232?ordinal, *Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273 (32) :20121-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9685355?ordina, *Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155 (3) :1229-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/7636191?ordina, *Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151 (11) :6470-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/8245480?ordina, *Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1) -induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66 (3) :1394-401., URL: https://www.ncbi.nlm.nih.gov/pubmed/1738199?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Applications Notes

Flow cytometry: Recommended dilution: 1-12 µg/ml

Isotype

Mouse IgG2a

Frequently Asked Questions

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