Anti-Hu beta2-Microglobulin Biotin
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Product Specifications
Certification
RUO
Reactivity
Pig, Human
Immunogen
Purified isolated human beta2-microglobulin.
Target Antigen
Beta2-Microglobulin
Clone
B2M-02
Applications
WB, IHC-P, ELISA, FC (QC tested)
Concentration
1 mg/mL
Format
Biotin
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98 (5) :1561-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11520808?dopt=, *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) . 1984;30 (6) :369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Applications Notes
Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
Isotype
Mouse IgG1