Anti-Hu beta2-Microglobulin Biotin

*Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37 (2) :139-45., URL: https://pubmed.ncbi.nlm.nih.gov/23146557/, *Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79 (24) : 15226-15237., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160, *Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21 (9) :1257-65. Epub 2006 Jun 30., URL: https://www.ncbi.nlm.nih.gov/pubmed/16810512?dopt=, *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) . 1984;30 (6) :369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A, *Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct;30 (14) :1223-30., URL: https://pubmed.ncbi.nlm.nih.gov/7692238/

The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.