Anti-Hu beta2-Microglobulin Biotin

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Product Specifications

Certification

RUO

Negative Species

Rabbit,Dog,Mouse,Cow,Chicken

Reactivity

Human

Immunogen

Purified human beta2-microglobulin

Target Antigen

Beta2-Microglobulin

Clone

B2M-01

Applications

FC (QC tested), IP, WB, IHC-P, ICC, ELISA

Concentration

1 mg/mL

Format

Biotin

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37 (2) :139-45., URL: https://pubmed.ncbi.nlm.nih.gov/23146557/, *Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79 (24) : 15226-15237., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160, *Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21 (9) :1257-65. Epub 2006 Jun 30., URL: https://www.ncbi.nlm.nih.gov/pubmed/16810512?dopt=, *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) . 1984;30 (6) :369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A, *Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct;30 (14) :1223-30., URL: https://pubmed.ncbi.nlm.nih.gov/7692238/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Applications Notes

Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.